KMUTT RESEARCH & DEVELOPMENT JOURNAL

Volume 42, No. 01, Month JANUARY, Year 2019, Pages 69 - 107

Antioxidant and anti-wrinkle activities of tubtim chumphae rice extract for application in facial cosmetics

Rittipun Rungruang, Jarasfah Modsuwan, Amornrat Srisukong, Nattapon Kaisangsri

Abstract Download PDF

The aim of this research was to analyze the contents of total phenolics, total flavonoids as well as biological activity of Tubtim Chumphae rice (TCR) extract for application in a cosmetic product. TCR was extracted with ethanolic solution. Total phenolics content, total flavonoids content and antioxidant activities of the extract were first investigated. It was found that the total phenolics and flavonoids contents were 80.20±0.03 gGAE/g extract and 8.18±0.05 gQE/g extract, respectively. IC50 of DPPH• and ABTS•+ were 0.77±0.006 and 0.29±0.002 mg/mL, respectively. The elastase and collagenase (MMP-1 and MMP-2) inhibition activities were 84.23±0.14%, 83.67±0.49% and 83.26±0.16%, respectively. Moreover, the toxicity to human skin fibroblast cells (CCD-986SK) was determined by the MTT colorimetric assay. The results indicated that TCR extract did not affect the CCD-986SK cells of which the survival of cells was 100.00±6.23%. The extract was then incorporated into a facial cream formulation, which was then tested for its stability under long-term and accelerated conditions for 3 months. Results showed that all the formulations were homogeneous upon the storage. Although the pH, viscosity and color of the products changed, such changes did not affect the product quality. Results suggested that ethanolic extract of TCR has a good potential for skin care product application.

Keywords

Tubtim Chumphae Rice / Phenolic Compounds / Biological Activity / Cosmetics

---