CHIANG MAI UNIVERSITY JOURNAL OF NATURAL SCIENCES

Volume 19, No. 01, Month JANUARY, Year 2020, Pages 34 - 51

Hom–kularb–dang rice bran extract for the prevention of uvb-damage against human skin fibroblast

Swanya Yakaew, Preeyawass Phimnuan, Khomsan Tiensomjitr, Wongnapa Nakyai, Nitra Nuengchamnong, Gareth Ross, Malyn Ungsurungsei, and JarupaViyoch

Abstract Download PDF

In this study, we investigated the protective effect of hydroglycolic crude extract of Thai red Hom–Kularb–Drice (HKD) rice bran on UVB-induced photoaging of human skin fibroblast. The HKD rice bran extract showed scavenging capacity with an EC50 of 4.37 0.34 mg/mL testing using a DPPH radical scavenging assay. The structural identification of proanthocyanidins compounds was performed by Liquid chromatography-electrospray ionization-quadrupole-time-of flight mass spectrometry (LC-ESI-Q-TOF-MS) in the hydroglycolic HKD extract. These compounds were cyanidin B2 type and (epi)catechin. The extract showed a no cytotoxic response was showed in human skin fibroblast. Furthermore, the HKD extract (20 μg/mL) exhibited a protective effect in UVB irradiated primary skin fibroblast, reducing MMP-1 (matrix metalloproteinases-1) expression and increasing production of type I procollagen. This data suggests that the HKD rice bran extract has potential for use as a skin anti-aging agent in cosmetic products.

Keywords

Hom–Kularb–Dang rice bran extract, UVB, Human skin fibroblast, MMP-1, Type I procollagen.

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