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ENIVRONMENT ASIA


Volume 13, No. 03, Month SEPTEMBER, Year 2020, Pages 70 - 79


The natural lipolytic yeast candida sp. rmutsb-27 isolated from pineapple for treatment of cooking oil contaminated wastewater

Pitchya Tangsombatvichit, Kanjana Pisapak, and Paweena Suksaard


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Some yeast species can be applied for treatment of oil-contaminated wastewater. Oil contamination is one of the most serious problems in agro-industrial countries. Fat, oil and grease (FOG) are waste products from household food preparation and industrial processing. The aims of this study are to isolate natural lipolytic yeasts from ripe pineapple and select for high lipase activity on Tween 80 agar. The result showed that yeast isolate No.27 had high lipase activity. It had oval-shaped cells with circular form, and the entire margin of the creamy colored colony had raised elevation and a rough surface. The isolate was identifi ed as Candida sp. using the ITS specifi c sequencing region and named Candida sp. strain RMUTSB-27. This isolation grew well on YP medium containing 2% cooking oil waste as well as 2% glucose at 32°C. For oil-contaminated treatment, 2% palm oil waste mixed with wastewater was added Candida sp. RMUTSB-27 showed signifi cantly reduction in pH, fat, oil and grease, chemical oxygen demand (COD), and total Kjeldahl nitrogen (TKN) for 4 days. Our results indicated that the yeast Candida sp. RMUTSB-27 is benefi cial for lipid uptake and degradation of oil-contaminated wastewater and could be utilized for future green environmental treatment.


Keywords

Ripe pineapple; Natural lipolytic yeast; Oil-contaminated wastewater treatment; Yeast Candida sp.



ENIVRONMENT ASIA


Published by : Thai Society of Higher Education Institutes on Environment
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