SONGKLANAKARIN JOURNAL OF SCIENCE & TECHNOLOGY

Volume 42, No. 04, Month JULY, Year 2020, Pages 941 - 947

Changes in secretory protein of porcine ampulla and isthmus parts of oviduct on follicular and luteal phases

Mayuva Youngsabanant and Wanna Mettasart

Abstract Download PDF

This study aimed to describe the morphology on oviductal epithelial cells (pOEC) and protein secretions in follicular and luteal phases. Result of proteins band were not different from control group. The fresh secretory proteins of ampulla and isthmus parts pOEC in the follicular phase were 45, 90, 105 kDa, while the fresh secretory fluids from ampulla and isthmus pOEC secretion in luteal phase were 38, 45, 90, 105, 220, >220 kDa. Protein of size 220 and >220 kDa was probably trypsin or protease functioning to stimulate ovulation. Protein sized 105 kDa is glycogen phosphorylase and protein kinase or oviductal glycoprotein is the enzyme for glycogenolysis plays an important role in regulation of glucose levels in the blood vessels. Protein of size 90 KDa was found to be heat shock proteins for inducing cell growth and in vitro fertilization development of spermatozoa. Protein sized 45 kDa is immunoglobulin gamma-chain function on oocyte maturation.

Keywords

porcine oviductal epithelial cells (pOEC), follicular and luteal phases, secretion proteins, SDS-PAGE, LC MS MS

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