SONGKLANAKARIN JOURNAL OF SCIENCE & TECHNOLOGY

Volume 42, No. 03, Month MAY, Year 2020, Pages 573 - 584

Mitochondrial dna diversity of the lemon emigrant butterfly catopsilia pomona fabricius (lepidoptera pieridae) in khon kaen province, thailand

Nutnicha Khomphimai, Chananchida Samranthin, Kanokporn Chaianunporn, Wibhu Kutanan, Thotsapol Chaianunporn

Abstract Download PDF

We investigated genetic diversity, population structure, and demographic history of a Catopsilia pomona population in Khon Kaen Province, Thailand, using partial 863 base pairs cytochrome oxidase subunit I (COI) sequences among various sampling locations and periods. We collected 10 butterfly individuals from 3 locations and from 2 periods, before migration (March to April 2017) and after migration (May to June 2017). The sequence analyses of 60 butterflies revealed 36 haplotypes defined by 36 polymorphic sites. In the six groups we studied, the haplotype diversity in each group was high (0.667–0.978), whereas the nucleotide diversity was relatively low (0.002–0.006). The haplotype diversity and the number of haplotypes increased after migration in all locations. The Analysis of Molecular Variance (AMOVA) showed no differences between the groups before and after migration (Φct=−0.030, P>0.9) and among sampling locations (Φct=−0.001, P>0.4). The Bayesian skyline plot indicated a previous demographic expansion since the Middle Pleistocene.

Keywords

cytochrome oxidase subunit I, genetic diversity, migration, mitochondrial DNA, population structure

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