The effect of rumen-protected rice bran oil (RP-RO) supplementation on performance and fatty acid content of
crossbred Wagyu beef steers was determined. Twelve crossbred Wagyu beef steers, that averaged 509±3.2 kg live weight and 28
months old, were stratified by their live weight into 3 groups. All steers were fed 7.5 kg/d of 14% crude protein concentrate with
ad libitum rice straw and had free access to clean water. The treatments were: 1) control concentrate; 2) supplemented with 100
g/d of RP-RO (100 RP-RO); and 3) supplemented with 200 g/d of RP-RO (200 RP-RO). This present study demonstrated that
supplementation of RP-RO did not influence dry matter and crude protein intakes, live weight changes, carcass and muscle
characteristics, and sensory or physical properties. RP-RO increased C18:1n-9 and beef marbling scores. Based on the results
from the present study, it can be recommended that the addition of 200 g/d RP-RO can increase C18:1n-9 and the beef marbling
score.