Yakae-Prajamduen-Jamod (ABP), which was acquired from Chao Phraya Abhaibhubejhr Hospital, is a traditional Thai
herbal formula to treat perimenopausal and menopausal symptoms. However, its mechanism remains unknown. The present
study aimed to investigate the effects of ABP on ovariectomy-induced anxiety and oxidative stress in mice. Ovariectomized mice
exhibited not only anxiety in elevated plus maze and mirror chamber tests, but also brain oxidative stress when compared with
control. Ovariectomy-induced behavioral and neurochemical alterations were attenuated by ABP treatment (500 mg/kg/day). We
also analyzed antioxidant activities of ABP using DPPH and ABTS assays. ABP showed radical scavenging activity with IC50
58.59 and 44.99 μg/mL, respectively. Total phenolic and flavonoid contents were 110.53±1.50 mg GAE and 152.25±0.5 mg rutin
equivalents per gram extract, respectively. These findings suggest that ABP attenuated the ovariectomy-induced anxiety and
oxidative brain injury via antioxidative properties. Therefore, ABP is an alternative choice of anxiety disorder in the menopausal
transition.