SONGKLANAKARIN JOURNAL OF SCIENCE & TECHNOLOGY

Volume 41, No. 06, Month NOVEMBER, Year 2019, Pages 1396 - 1403

Investigation of biodegradable chitosan nanoparticles incorporated with gd-dtpa prepared by ionotropic gelation for magnetic resonance imaging

Waralee Watcharin and Suchawadee Wiratthikowit

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A nanoparticle based magnetic resonance imaging (MRI) contrast agent was studied using the biopolymer chitosan. (Gd-DTPA)-conjugated chitosan nanoparticles were prepared by a one-step ionotropic gelation method at chitosan concentrations of 0.5 and 1.0 mg/mL. The nanoparticles were characterized by scanning electron microscopy and the particle size, zeta potential, and polydispersity index (PDI) were measured by dynamic light scattering. The results showed that (Gd-DTPA)-conjugated chitosan nanoparticles were homogeneous and the size had a narrow distribution based on the PDI which was in the range of 0.1– 0.3. The mean±SD of the Gd content was found to be 6.2±1.5 mg/g particle. The in vitro release study along with UV-vis spectra showed no free Gd ions released from the prepared nanoparticles. The results confirmed that the binding of Gd-DTPA to chitosan did not exert any influence on the nanoparticle size or zeta-potential.

Keywords

nanoparticles, chitosan, Gd-DTPA, MRI contrast agent

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