SONGKLANAKARIN JOURNAL OF SCIENCE & TECHNOLOGY

Volume 41, No. 05, Month SEPTEMBER, Year 2019, Pages 1029 - 1036

Porcine cumulus oocyte complexes (pcocs) as biological model for determination on in vitro cytotoxic of cadmium and copper assessment

Waramanee Pongsawat, Mayuva Youngsabanant

Abstract Download PDF

We demonstrated the potential of primary porcine cumulus oocyte complexes (pCOCs) used as a biological model for determination of in vitro toxicity of cadmium and copper. After treatment at a concentration of 10-4 M of cadmium and copper for 44 h, the surrounding cumulus cells (CCs) stopped the expansion process and the pCOCs did not undergo maturation in either treatment. Compared to the control, degradation of the pCOCs was observed as well as a number of vacuoles associated with metal accumulation. Cumulus cells and zona-pellucida revealed a rough surface with numerous knobs. The cumulus cells showed a loss of cell margin and signs of cytoplasmic organelle damage. The percent adsorption rate of cadmium and copper from inductively coupled plasma were 5.65 and 6.85, respectively, and there was no significant difference between treatment groups (P>0.05). This finding is valuable for future cell technology research in medical and in vitro cytotoxicity studies.

Keywords

cadmium, copper; cytotoxicity, porcine cumulus oocyte complexes (pCOCs), electron microscopy

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