SONGKLANAKARIN JOURNAL OF SCIENCE & TECHNOLOGY

Volume 43, No. 01, Month JANUARY, Year 2021, Pages 72 - 79

Influence of stabilizers and cryoprotectants on the characteristics of freeze-dried plga nanoparticles containing morus alba stem extract

Kanyarat Srihaphon, Supaporn Lamlertthon, Tasana Pitaksuteepong

Abstract Download PDF

This study aimed to investigate the effects of stabilizers and cryoprotectants on the characteristics of poly (lactic-coglycolic) acid (PLGA) nanoparticles containing Morus alba stem extract (MSE). The stabilizers used in the preparation of the nanoparticles were polyvinyl alcohol (PVA) and poloxamer 188 at a concentration of 2% w/v. The effects on freeze-drying of cryoprotectants, including glucose, sucrose, maltose and mannitol at concentrations of 5% and 10% w/v, were investigated. The results showed that the presence or the absence as well as the type of stabilizer affected size and charge of the nanoparticles. They also affected the loading capacity and the release of MSE. In freeze-drying, the type of cryoprotectant greatly influenced the physical stability of the nanoparticles and the chemical stability of encapsulated MSE. The nanoparticles prepared using 2% w/v PVA as the stabilizer and freeze-dried in the presence of 5% sucrose were the optimal carriers of MSE.

Keywords

Morus alba stem extract, PLGA, nanoparticles, stabilizer, cryoprotectant

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