SONGKLANAKARIN JOURNAL OF SCIENCE & TECHNOLOGY

Volume 41, No. 01, Month JANUARY, Year 2019, Pages 37 - 44

Growth profile and partial characterization of bacteriocin produced by lactobacillus plantarum atm11 isolated from slaughterhouse soil

Alagu Thirumurugan, Rajajurn Sheela, Amaresh Kumar Singh

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In this study, we report on the growth profile and partial characterization of a bacteriocin produced by Lactobacillus plantarum ATM11 isolated from goat slaughterhouse soil. Among eight bacteriocin-producing bacteria from 25 Lactobacilli isolates of goat slaughterhouse soil, the higher-yielding bacteria were selected and identified as Lactobacillus plantarum based on the morphology, biochemical characteristics, and partial 16S rRNA gene sequence. The physico-chemical condition effects of temperature, pH, enzymes, and detergents of bacteriocin activity were studied. The optimum temperature for bacteriocin activity was found to be 60 C. The optimum pH for bacteriocin activity was found to be in the range of 5.0-6.0. Detergents such as sodium dodecyl sulphate (SDS), Triton X-100, and ethylene diamine tetra-acetic acid (EDTA) were influenced the bacteriocin activity, whereas urea inhibited the activity. Asparaginase and -amylase enzymes influenced the activity, whereas protease, proteinase K, and lysozyme inhibited bacteriocin activity. The present study revealed that the action mode of the produced bacteriocin has a bacteriostatic effect against Bacillus cereus MTCC 1272 and Micrococcus leuteus MTCC 2987. Thus, it can be used as a bio-preservative agent for different foods in the future.

Keywords

bacteriocin, biochemical tests, 16S rRNA sequencing, L. plantarum ATM11, growth profile, physicochemical characterization

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