SCIENCE ASIA

Volume 47, No. 06, Month DECEMBER, Year 2021, Pages 751 - 757

Development of a new efficient scar marker for authentication of angelica sinensis, an important traditional chinese medicine

Hua Peng, Guo Chen, Lilan Ou, Mao Luo, Chun Zhang

Abstract Download PDF

Angelica sinensis is a well-known traditional Chinese food and medicine that is often misused or intentionally substituted on the market. DNA samples from 6 A. sinensis cultivars and 23 related species were used to develop an efficient sequence-characterized amplified region (SCAR) marker. Eighteen inter simple sequence repeat (ISSR) primer-amplified bands were highly polymorphic (83.0%). The ISSR primer UBC826 amplified a specific fragment of 899 bp (GenBank accession no. KP789480) only from A. sinensis. The fragment was eluted, purified, cloned, and sequenced. One set of SCAR primers (UBC826 L/UBC826R), developed from the sequence information of the fragment, was found to be specific to A. sinensis and produced an amplicon of 749 bp in size. Furthermore, a new method for DNA extraction of Chinese patent medicines was developed, and 5 Chinese patent medicines containing A. sinensis were used for efficient detection of the A. sinensis-specific marker. The ISSR-based SCAR marker was developed and easily tested by PCR amplification. This marker provides an effective and precise PCR-based diagnostic method and might be conducive to raw material production and quality control for A. sinensis.

Keywords

Angelica sinensis, ISSR, molecular authentication methods, SCAR

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