Introduction: Payena lucida A.DC. seed oil has long been used as edible oil and treating
various kinds of diseases as oral and topical applications in some local areas of Myanmar.
Objectives: This research aimed to identify and qualify the physicochemical characters and
fatty acid composition of P. lucida seed oil. Materials and Methods: Two authentic seed oils
of P. lucida were prepared and evaluated in comparison with two commercial seed oils. The
physicochemical characters of the seed oils were determined by methods described in the USP
39, and the fatty acid composition was analyzed by gas chromatography-mass spectroscopy.
Results: The physicochemical characters of authentic and commercial seed oil samples were
not much different. Their specific gravities, refractive indices, acid values, saponification values,
ester values, and iodine values were 0.9117–0.9138, 1.4645–1.4652, 3.88–12.03 mg KOH/g,
210–213 mg KOH/g, 199–208 mg KOH/g, and 68.85–84.85 g I2/100 g, respectively. The amount
of saturated, monounsaturated, and polyunsaturated fatty acids were 38–41%, 53–55%, and
7–8%, respectively, with oleic acid (53–55%) as the major fatty acid. Conclusions: Quality of
P. lucida seed oil was concluded in terms of physicochemical characters and fatty acid composition.
Commercially available seed oils showed comparable quality with those of authentic seed oils.